The spread of public service advertisements of tobacco control is of kind dissemination of healthy culture，for these advertisements attempt to change the attitude and behavior of publics through artistic form，and they imperceptibly influence the values and standards of behavior of publics.In this paper，the empirical study methods is used to learn the most often used media in new media era，and explore and like of publics on Public Service Advertisements of Tobacco Control.Furthermore，this paper analyzes the acceptability of publics of form，content and conception of this kind of advertisement，and it studies the effects of different kinds of advertisement.The research results offer strong evidence for government，media and other relevant institutions who will construct the Public Service Advertisements of Tobacco Control.