This chapter，firstly based on the three perspectives of science and technology innovation giving basic support to city innovation and transformation，enhancing economic growth as the engine，and promoting city function，analyzed the function and effect that science and technology innovation and transformation on city development. On this basis，combined with the actual situation in Shanghai，this chapter designed and constructed the comprehensive evaluation system which reflects Shanghai innovation，transformation and development，measured Shanghai innovation and transformation of development index during 2006-2013. This chapter also summed up the experience of city transformation and innovation in developed countries，and the significance reference for Shanghai. At last，this chapter compared and analyzed the science and technology innovation ability of the main cities in the Yangtze River Delta in 2013，then made key comparison on the technological innovation mode of Shanghai and Shenzhen，and summarized its enlightenment to Shanghai.