The Internet Cultural Literacy consists of four dimensions：Internet scientific and cultural literacy，Internet traditional cultural literacy，Internet red cultural literacy，and Internet media information literacy. This report collected sample data through a quota sampling method，combining online and offline channels. The results showed that Shenzhen netizens’ average Internet cultural literacy score was 80.4 points，Internet scientific and cultural literacy score was 78.5 points，Internet traditional cultural literacy score was 81.9 points，Internet red cultural literacy score was 79.7 points and Internet media information literacy score was 81.7 points. In general，the Internet culture literacy of Shenzhen netizens is satisfactory. Shenzhen netizens are good at searching，understanding and evaluating information through the Internet，and have strong abilities for independent online learning. Meanwhile，they also demonstrate a strong awareness，identification and willingness to engage with red culture and Chinese traditional culture. A higher level of Internet users’ cultural literacy not only helps Internet users with self-improvement，but is also of great significance to the transmission and development of mainstream culture in the new era.