The law of People’s Republic of China on the National Economic and Social Development of 14th Five-year Plan and 2035 Vision Outline Planning Deployment，give full play to the innovative city to achieve high levels technology of free-standing self-improvement and the construction of the strategic pivot role of science and technology power，full swing high level national innovative city construction. So far seventy-eight cities （districts） in China have been rated as national innovative cities，and five cities in Northeast China have been rated as national innovative cities，including Shenyang，Dalian，Changchun，Jilin and Harbin. In this report，according to National Innovative City Innovation Ability Evaluation Report 2021，the national innovative cities in Northeast China are classified and evaluated，and respectively to innovation governance，original innovation，technology innovation，achievement transformation and innovation driving force of five aspects of in-depth analysis and evaluation. The innovation ability of the five innovative cities in northeast China is different. Dalian’s innovation governance ability，technological innovation ability and innovation driving force rank first in northeast China. The transformation of Shenyang’s achievements is outstanding in northeast China. Changchun’s original innovation capacity far exceeds other cities in northeast China. Further application of data envelopment method （DEA） analysis of a national innovative city in the northeast of input and output efficiency of science and technology innovation and the national innovative city in northeast Chin has yet to reach full effective，and there are large difference between science and technology innovation achievements conversion rate. Finally，according to the problems existing in the ability of science and technology innovation of national innovative cities in northeast China，the relevant countermeasures and suggestions are put forward，such as for establishing cross-regional cooperation platforms，promoting collaborative innovation development，enhancing the development of high-tech industrialization，and increasing basic research investment.