With the advancement and development of modernization，the spatial pattern of “living-production-ecological spaces” in the urban agglomerations has changed. This paper takes the middle reaches of Yangtze River urban agglomeration as the research object and conducts a quantitative study on the evolution of the spatial pattern of “living-production-ecological spaces” at macroscopic and microscopic scales from 2000 to 2020 by means of dynamic degree，standard deviation ellipse，landscape pattern analysis and LEI index. The main conclusions are as follows：①the living space of the middle reaches of Yangtze River urban agglomeration has remained stable over the past 20 years，while the production space has gradually expanded and the ecological space has fluctuated and decreased. ②The area transformation of agricultural production space，woodland ecological space and water ecological space is the largest among the living-production-ecological spaces. ③The long axis of the standard deviation ellipse of all three types of space in 20 years is northwest-southeast direction，and the production and ecological space are opposite to the expansion direction of living space；there are differences in the changes of the closeness of the three types of space；the evolution trends of production and living space in different metropolitan areas are different.