Ecotourism is the main function of the National Park model. The development of ecotourism in national parks involves complex stakeholder relationships. Based on the symbiosis theory，this study takes Wuyishan National Park as an example to analyze and discuss the demands and relationships of tourism stakeholders in the development of National Park tourism. The research finds that：firstly，The stakeholders of ecotourism in national parks show common interest demands in protecting ecological and human resources and developing high-quality ecotourism；secondly，The right matrix of National Park ecotourism stakeholders is unbalanced，and interest alliances and confrontation are easy to form in competitive games and cooperative games；thirdly，The government，enterprises，residents，National Park Administration and operators tend to choose different ways to realize their interests. Therefore，it should continue to explore the conditions，model and realization mechanisms of interest symbiosis according to the interest demands and game status of stakeholders in the development of National Park ecotourism.