Grassland culture is an important part of the Yellow River culture. The grassland culture of the Yellow River basin is concentrated in the upper and middle reaches of the Yellow River，including Anduo culture，Hehuang culture，Longyou culture，Hetao culture and so on. Grassland ecological culture is the essence of grassland culture，which reflects the ecological wisdom of respecting nature，conforming to nature and protecting nature. At present，we should further protect and explore the spiritual connotation of the grassland culture of the Yellow River basin，carry forward its value of the times and promote the innovative development and industrial development of grassland ecological culture in the Yellow River basin.