as an important science popularization infrastructure，science and Technology Museum is an important carrier of national science popularization ability. The rapid development of short video in recent years has attracted the attention of science and technology museums in China. The science and technology museum can use the short video platform to popularize scientific knowledge，carry forward the scientific spirit，spread scientific ideas and advocate scientific methods to the general public，especially young people，in a more intuitive and vivid form，which is conducive to overcoming the limitations of the physical museum and expanding the audience of the science and Technology Museum. The dissemination of short videos of science and Technology Museum is a new attempt to innovate the communication mode of science and Technology Museum and explore the combination of online and offline，entity and virtual. Therefore，the characteristics of science and Technology Museum in the form of short videos on the Tiktok platform are very noteworthy. This study conducted an overall survey and sample crawling of all official accounts above the provincial level on the Tiktok platform（265 in total）. Based on the analysis，this paper combs the current situation of short video content and effect of science and Technology Museum，and summarizes the characteristics and existing problems.