Expanding the scope of public interest litigation cases is not only the resolution of the Fourth Plenary Session of the 19th Central Committee，but also the key work of public interest litigation by procuratorial departments in recent years. Through empirical analysis of public interest litigation cases involving protection of specific groups’ rights and interests handled by procuratorial organs，it can be seen that this kind of public interest litigation is different from the traditional public interest litigation in “five major areas”，which is characterized by civil litigation，and administrative public interest litigation is mainly based on pre-litigation procedures. This paper selects the procuratorial public interest litigation of protecting women’s rights and interests as the breakthrough point，and emphatically analyzes the social，public and orderly characteristics of the interests protected by this kind of litigation. This paper analyzes the difficulties existing in legislation and law enforcement of the protection mechanism of women’s rights and interests，demonstrates the necessity of constructing the procuratorial public interest litigation mechanism of women’s rights and interests protection，and finally points out the feasible path of constructing procuratorial public interest litigation of women’s rights and interests protection.