Construction of national independent innovation demonstration zone is an important measure to speed up the construction of innovative country and the implementation of innovation driven development strategy. November 3, 2014, South of Jiangsu national independent innovation demonstration zone approved, becoming the second national strategy in Jiangsu province. In this paper, the South of Jiangsu national autonomous innovation demonstration zone to promote the construction of a breakthrough in Jiangsu ideas, focus and countermeasures. This paper first discusses the status quo and problems of the construction of South of Jiangsu national innovation demonstration zone; and then finishing the South of Jiangsu to promote the independent innovation demonstration zone construction strategy and key breakthrough, should be pointed out that according to the strategic positioning of the national "Three District Heights", to deepen the reform of institutional mechanisms, strengthen the innovation of leading function, strengthen the development of regional innovation the integration of open innovation and strengthen the function of ideas, focus on breakthrough from six aspects to promote the reform of science and technology, establish the dominant position of enterprise innovation, the key breakthrough in core technology, carry out regional collaborative innovation, the development of science and technology services and promote open innovation and cooperation; and then puts forward countermeasures of South of Jiangsu innovation demonstration zone construction, is a is the prominent regional linkage, the formation of independent innovation ability; two is to highlight the dominant position of enterprises, construction level Group of rich of independent innovation of enterprises; the third is the prominent industry oriented, to create innovative industrial clusters; the fourth is highlighting the basis of support, the construction of the carrier to accelerate innovation, leading the outstanding talent, innovation and entrepreneurial talent agglomeration; six is highlight the advantages of the open, promote innovation and international cooperation; 7 is the prominent reform guidance, speed up institutional innovation; eight is outstanding service facilities, improve the ecological environment of innovation. <<