Difference is a distinctive characteristic of “One Belt and One Road”. “One Belt and One Road” includes Asia，Africa and Europe that are distant in geography and have great differences in cultures and development levels. The supplies and demands of China are very consistent with those of Africa，Asia and Europe. The difference is a premise of complementarity，the bigger the difference，the greater the complementarity. For the cooperation among “One Belt and One Road” countries，we need find accurate positioning，give play to advantages and keep the differences in the differences. Difference is an important factor to maintain the balanced development and the industrial isomorphism is likely to cause vicious competition.