The effective evaluation on international trade cooperation value with China for the main countries along the “Yu-Xin-Ou” Railway route shows high significance for the selection on targeting countries，the upgrading of China’s industrial structure，and the sustainable development of China’s foreign trade. The international trade cooperation value is depending on three key factors，including trade facilitation，trade market environment，and bi-lateral trade complementary level. The route countries along “Yu-Xin-Ou” Railway has shaped a “west-high，east-low” geographical pattern for their international trade cooperation value. The calculation based on trade complementarity index proves that the route countries have high bi-lateral trade complementary level with China，indicating that in future China could strengthen these bi-lateral trade relations and further push forward the cooperation into a new stage.