In the context of “One Belt and One Road”，the pace of Chinese enterprises going out is getting faster，and interaction with overseas Chinese and Chinese overseas have become increasingly close. This paper discusses the interaction between Chinese enterprises and overseas Chinese and Chinese overseas recently. Also，analyze the possible opportunities and challenges in this relationship in the aspects of politics，economy and culture. The paper makes the mechanism of interactive relationship between overseas Chinese，Chinese enterprises，the countries and China. In the end，the paper provide some recommendations for the interaction between overseas Chinese and Chinese enterprises going out in the future：premise on the basis of mutual tolerance，establish beneficial cooperation mutually，construct a full rage of platform for comprehensive cooperation and build a worldwide network for interaction.