“One Belt and One Road （OBOR）” initiative is China’s active participation in the top-level design of global and regional governance in the 21st century，and the trade flow is one of the “five connectivity” of the initiative，which is the key content of the construction of “OBOR”. This study analyzes the problem of the trade flow between China and the countries along the six economic corridors of the “OBOR” from the perspective of cultural differences. Using the relevant data of 64 countries along the six economic corridors of the “OBOR” and China in 2007-2017 as the analysis sample，taking the variables of port infrastructure quality，customs formalities burden，international tourism revenue and expenditure，education input level as the control variable. Empirically study the influence of cultural distance，geographical distance and economic scale on the trade flow between China and the six economic corridors through an extended trade gravity model. In addition，this paper compares and analyzes the trade convenience between economic corridors and China through political，economic and cultural perspectives，and further discusses the role of overseas Chinese in the smooth trade between China and the countries along the Belt and Road.