With the great enrichment of urban residents’ material life，cultural life based on spiritual satisfaction has gradually become an important symbol of residents’ healthy living. Scientific evaluation of urban residents’ cultural life is of great significance to improve the healthy life quality of urban residents. This report expounds the concept of urban culture，the importance and the significance of cultural evaluation，on the basis of the existing evaluation indexes at home and abroad for reference，Seven indexes were selected from three aspects of cultural input，education level and cultural facilities to construct the evaluation index system of the healthy living culture of urban residents in China，evaluate the country’s 289 cities environment condition，and the evaluation results are analyzed in depth.