“Three cities and one district” is the main platform for Beijing to build a national science and technology innovation center，which provides a good support for the development of high-tech industries. The popularization of major scientific and technological achievements of the “Three Cities and One District” main platform will greatly satisfy the public’s need for awareness，understanding，familiarity and even use of high-end scientific and technological resources. This article summarizes the science popularization work carried out in the “three cities and one district” in the past three years，analyzes the challenges facing the internationalization of science popularization in the “three cities，one district”，and points out the limitations of the internationalization of science popularization in the “three cities and one district”. The countermeasures and suggestions for the internationalization of science popularization in Beijing’s “three cities and one district” are put forward.