The “space-time compression” effect produced by high-speed rail has an important impact on the regional tourism spatial pattern of the Yangtze River Economic Belt. This paper uses the time cost grid method to measure the change of traffic accessibility and other time zones of some typical cities and tourism resource points in the Yangtze River Economic Belt without high-speed rail and high-speed rail，and explores the impact of high-speed rail on the regional tourism spatial pattern of the Yangtze River Economic Belt. The results show that：（1）The core cities of the five major urban agglomerations in the Yangtze River Economic Belt are all expanding outward under the conditions of high-speed rail. The range of AC radiation in urban agglomerations is expanding under the influence of high-speed rail. （2）The high-speed rail has greatly improved the market size and economic level of the tourist resources in the region，and strengthened the spatial connection of inter-regional tourism flows. （3）The high-speed rail has brought the O-D time and space distance between the city and the tourism resource points，and strengthened the spatial and temporal correlation between the tourist source and the tourist destination of the Yangtze River Economic Belt.