Cultural industry is an important support for transforming economic growth mode and enhancing the country's soft power under the new economic normality. In view of the continuously deepening integration of cultural industry in depth and breadth，this report discusses the development law of cultural industry from the perspective of cultural industry ecosphere. Based on the theory of business ecosystem，this paper firstly constructs the micro-structure model of cultural industry ecosphere from four subsystems：industrial core，external support，macro-environment and industrial competition. It holds that cultural industry ecosphere is a nested network system with regional and industrial levels，as well as self-organization characteristic. Based on the different characteristics of the cultural industry ecosphere at different stages of life cycle，the leading roles of the government and the market are also characterized by stages. Finally，based on the above theoretical analysis，this report uses the successful experience of cultural industry development in South Korea and the United States to verify the micro-structure model of cultural industry ecosphere.