Hong Kong-owned enterprises epitomize and witness the integration of Guangdong and Hong Kong. As a “third kind of capital” different from “pure foreign capital” and domestic investment，Hong Kong capital has unique research value. Based on the theoretical perspective of gender temperament，this paper uses the data from the PRD firms to investigate the cultural motivation of Hong Kong-funded enterprises' patent innovation by using the negative binomial regression model. The following conclusions are drawn：The innovation performance，co-innovation performance and femininity of Hong Kong-owned enterprises are better than non-Hong Kong assets；the femininity has a significant positive impact on the innovation performance of both Hong Kong-owned and non-Hong Kong-owned enterprises. The synergistic innovation behavior plays a partial mediating effect in the influence of femininity on the patent innovation performance of Hong Kong-owned and non-Hong Kong-owned enterprises，but the intermediary effect is small，and the influence of femininity on patent innovation performance mainly takes the direct effect；Compared with non-Hong Kong-owned，Hong Kong-funded enterprises have more effective intermediary of collaborative innovation.