The Ming’s Yi Culture is formed in the collision and blending of science，the philosophy of mind and Buddhism and it reflects to the Idea and characteristics of academic and cultural development in this period. The set of Ming Dynasty is limited to one standard and the Yi culture made the Cheng Zhu school’s learning as the authentic theory. However，in the Ming Dynasty at the time of prevalence of Cheng Zhu School，there was another school completely different and even opposed to Cheng Zhu：one is the Yi culture in the field of philosophy of mind and another one is the agro meteorology that aimed to restore the textual research tradition in the Han Dynasty. The Ming’s Yi Culture experienced from the Cheng Zhu School’s Domination，to the correction and critics of Cheng Zhu，that is to interpret “Yi” with the philosophy of mind and Buddhist，then turned to reconcile the science and philosophy of mind and the intermediation of Confucianism and Buddhism. This makes “Yi” in this period with the characters of fusion of agrometeorology and argumentation philosophy of mind and science，Taoism and Confucianism. The value of Ming’s Yi is to establish the legal authority of Cheng Zhu’s learning in the Ming and Qing Dynasties，integrate the Song’s Yi that made the Cheng Zhu’s learning as the mainstream，and fix its flaws in some respects，them form its own concepts of Yi. It greatly promoted the development of Yi and is an important and indispensable step in the history. Although in the Qing Dynasty it received some criticism，we should not totally deny the Ming Dynasty’s academic contribution to Yi.