The development of blood transfusion in China began in 1918 when Shanghai first reported the Chinese blood type，and it has been 100 years old.In order to ensure the safety of blood transfusions，in particular to prevent transmission of the virus，a series of measures have been taken among which blood screening for viral markers have played an important role in and guarantee the security of transfusion，including hepatitis B virus surface antigen，hepatitis C virus antibody，HIV antibody，and syphilis.These viral infection indicators are also mandatory infectious screening items that are explicitly mentioned in the Blood Screening Proposal of WHO.Since 1960s，blood stations around China have carried out tests for HBsAg，HCV，HIV，and TP one after another，which has led to a significant drop in the transmissible virus infection rate.In our country，Elisa reagents have been widely used as blood screening reagents in the nationwide blood collection and supply system.That have the advantages of low cost，short reaction time，simple operation，and low requirements on the environment and equipment.It played an important role in protecting China's blood safety.This article aims to provide an overview of the application history in blood stations of the four-important blood screening Elisa reagents for HIV，HBV，HCV and TP.